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In Situ Soil Cementation With Ureolytic Bacteria By Surface

This study proposes an approach for applying biocementation in situ by combining the surface percolation of nutrients and cementation solution ureaCaCl 2 with in situ cultivation of indigenous soil urease positive microorganisms under nonsterile conditions The enrichment of indigenous ureolytic soil bacteria was firstly tested in batch

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Surface Percolation For Soil Improvement By Biocementation

This study proposes an approach for applying biocementation in situ by combining the surface percolation of nutrients and cementation solution ureaCaCl 2 with in situ cultivation of indigenous soil urease positive microorganisms under nonsterile conditions The enrichment of indigenous ureolytic soil bacteria was firstly tested in batch

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Stimulating In Situ Soil Bacteria For Biocementation Of

Mar 25 2014nbsp018332Stimulating In Situ Soil Bacteria for BioCementation of Sands A research program was implemented to assess the ability of biostimulation treatment solutions to stimulate native ureolytic bacteria in a variety of soils ranging from quarried sands to lacustrine siltysand deposits using soil specimens prepared in 102 cm high by 51 cm

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Biogrout A Novel Binding Material For Soil Improvement

In situ soil cementation with ureolytic bacteria by surface percolation Ecol Engin 42 6472 101016 Applications of microorganisms to geotechnical engineering for bioclogging and biocementation of soil in situ Rev Environ Sci Study on microstructure and properties of sandstone cemented by microbe cement Const Build

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Investigating Ammonium Byproduct Removal For Ureolytic

In this study five 37meter long soil columns were improved using ureolytic biocementation and posttreatment NH 4 byproduct removal was investigated as a function of different soil

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Surface Percolation For Soil Improvement By Biocementation

Cheng L and Shahin M and CordRuwisch R 2017 Surface Percolation for Soil Improvement by Biocementation Utilizing In Situ Enriched Indigenous Aerobic and Anaerobic Ureolytic Soil Microorganisms Geomicrobiology Journal 34 6 pp 546556

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Effect Of Microbially Induced Calcite Precipitation On

Sep 11 2019nbsp018332In situ soil cementation with ureolytic bacteria by surface percolation Ecological Engineering 42 64 72 2 Cheng Liang and Ruwisch Ralf Cord 2014 Upscaling Effects of Soil Improvement by Microbially Induced Calcite Precipitation by Surface Percolation Geomicrobiology Journal 31 396406

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Isolation Differentiation And Biodiversity Of Ureolytic

221 Enrichment of ureolytic bacteria Sterile syringe barrels of 30 mL were filled with soil and enrichment medium g L 1 025 glucose 138 sodium acetate 05 Bacto yeast extract and 20 filtered urea up to 20 mL of volume for each sampleThe enrichment solution was modified from the method used by Burbank et al 2012 5 A tube was connected to each syringe and was closed by clamp

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Soil Biocementation Using A New Onephase Lowph

Oct 17 2018nbsp018332Soil biocementation via microbially induced carbonate precipitation MICP has been extensively studied as a promising alternative technique to traditional chemical cementing agents for ground improvement The multiplephase injection methods are currently well adopted for MICP treatment but it is rather complex and requires excessive number of injections

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High Strength Insitu Biocementation Of Soil By Calcite

The maximum insitu urease activity was achieved by supplementing the cementation solution with growth media and the use of 05 M urea and Ca2 as cementation solution The insitu urease activity differed according to the different bacterial strains which tolerated the cementation conditions differentlyud ud One of the advantages of the

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Upscaling Application Of Microbial Carbonate

Jul 20 2016nbsp018332AlThawadi S 2008 High strength insitu biocementation of soil by calcite precipitating locally isolated ureolytic bacteria Phd Thesis Murdoch University AlThawadi S CordRuwisch R Bououdina M 2012 Consolidation of sand particles by nanoparticles of calcite after concentrating ureolytic bacteria in situ

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Soil Bacteria Population Dynamics Following Stimulation

Microbialinduced CaCO3 precipitation MICP via ureahydrolysis ureolysis is an emerging soil improvement technique for various civil engineering and environmental applications Insitu application of MICP in soils is performed either by augmenting the site with ureolytic bacteria or by stimulating indigenous ureolytic bacteria Both of these approaches may lead to changes in the indigenous

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Consolidation Of Sand Particles By Nanoparticles Of

MCP11 DSM 23526 were successfully concentrated in situ by uploading 3void volumes of cells followed by cementation solution calciumurea Sandstone with UCS of 1200 kPa was formed with three applications of cells and cementation solution without clogging the void spaces resulting in a permeable consolidated sand core

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Insitu Microbially Induced Ca2alginate Polymeric

The soil zone treated with the insitu formed Caalginate gel could be a promising method to control the seepage of contaminants to the surrounding environment and manage the contaminants migration in the underground space As a promising remedial manner further systematic evaluation on the current approach for heavy metals and organic

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Microbially Induced Sand Cementation Method Using

difcult to control the growth rate and induce uniform soil cementation using this technique Sporosarcina pasteurii which produces highly active urease is commonly used for soil improvement by bacteria21736 This ureolytic bacterium stimulates urea hydrolysis which leads to production of CaCO 3

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Frontiers Biogrout A Novel Binding Material For Soil

Mar 17 2016nbsp018332In situ soil cementation with ureolytic bacteria by surface and Chu J 2008 Applications of microorganisms to geotechnical engineering for bioclogging and biocementation of soil in situ Rev Rong H Qian CX and Li LZ 2012 Study on microstructure and properties of sandstone cemented by microbe cement

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Biological Process Of Soil Improvement In Civil

Oct 01 2016nbsp018332In situ soil cementation with ureolytic bacteria by surface percolation May 1 2012 183 Ecological Engineering 341 1 Liang Cheng Murdoch University HIndex 13

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Calcium Carbonate Crystals Formation By Ureolytic Bacteria

Ureolytic bacteria were isolated selectively from sludge and soil samples Perth Western Australia Three isolates were genetically examined by 16S rRNA They were mostly closely related to Bacillus species A bacterium producing the highest urease activity Bacillus sp MCP11 DSM 23526 was used to precipitate calcium carbonate crystals

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Enhancing Strength Of Micptreated Sandy Soils From Micro

Cheng L and CordRuwisch R 2012 In situ soil cementation with ureolytic bacteria by surface percolation Ecological Engineering 42 6472 Cheng L Shahin M A and Mujah D 2017 Influence of Key Environmental Conditions on Microbially Induced Cementation for Soil Stabilization Journal of Geotechnical and

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Evaluating The Potential Of Native Ureolytic Microbes To

Soil Bacteria Population Dynamics Following Stimulation for Ureolytic MicrobialInduced CaCO3 Precipitation Environmental Science amp Technology 2016 50 2 616624 DOI 101021acsest5b04033 George Redden Don Fox Chi Zhang

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Stiffness And Strength Of Granular Soils Improved By

Cheng L Shahin M A CordRuwisch R 2017 Surface Percolation for Soil Improvement by Biocementation Utilizing In Situ Enriched Indigenous Aerobic and Anaerobic Ureolytic Soil Microorganisms Volume 34 2017 Issue 6 pages 546 to 556 DOI 1010800149045120161232766

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Fly Ash Incorporated With Biocement To Improve Strength Of

Feb 07 2018nbsp018332This MICP process is dependent on ureolytic bacteria detector that detailed surface composition FTIR spectra of soil specimens were In situ soil cementation with ureolytic bacteria

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Carbonate Precipitation Through Microbial Activities In

Jan 20 2016nbsp018332Furthermore the efficiency of ureolytic bacteria to bind various types of heavy metal ions is due to their large surface area and availability of anions on the cell surface Wong 2015 Soils and groundwater contaminated with radionuclides particularly 137 Cs and 90 Sr pose a longterm radiation hazard to human health

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Indepth Profiling Of Calcite Precipitation By

Ureolytic bacteria produced homogenous inorganic fine crystals whereas the crystals of nonureolytic strains were larger and had a mixed organicinorganic composition When representative strains were tested in application for crack healing in cement mortars nonureolytic bacteria gave robust results while ureolytic strains showed more variation

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Strengthening Water Retention Capacity Of Marine Soft Clay

Biocement is biologically produced salt largely calcium carbonate along with the cell mass of microorganisms It is mainly generated by urease producing organisms The present study focuses on the feasibility of using biocement to strengthen the marine soft clay in order to increase its water retention capacity Eight urease producing organisms were initially isolated from sewage and

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